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Antibodies sure to the blocking peptide not bind to the epitope to the goal protein. By evaluating the staining within the blocked antibody as opposed to the antibody by yourself, you can see which staining is distinct.
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This guidebook outlines additional doable optimization of mobile and immunoassay parameters to ensure the very best benefits are...
This guide outlines even more probable optimization of cellular and immunoassay parameters to guarantee the very best outcomes are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind exclusively on the focus on antibody and block antibody binding. These peptide typically is made up of the epitope regarded because of the antibody.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic party. Cyclin E types a fancy with and functions as a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S get more info phase.
Overexpression of the gene has become observed in lots of tumors, which ends up in chromosome instability, and so may contribute to tumorigenesis. This protein was located to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant purpose in promoting mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two additional splice variants ended up documented but detailed nucleotide sequence information and facts is just not however obtainable.
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